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Optimize your Cell Culture Media Performance With CQREX® Ingredients
CQREX® INGREDIENTS ENSURE QUALITY, CONSISTENCY REDUCE RISKS AND IMPROVE PRODUCTIVITY
Our cQrex® portfolio of peptides is designed to overcome limitations in media formulation and storage stability. Our peptides also help to intensify cell culture processes and drive overall productivity by solving the common stability and solubility challenges of amino acids.
Here are some ways cQrex® can help you improve your cell culture systems.
- To maximize consistency, you can use defined components instead of non-defined complex mixtures to remove a source of risk related to batch-to-batch variation.
- To eliminate the negative effects of excessive salt and pH spikes, you can use soluble peptides that do not require the use of caustic feeds to solubilize sufficient nutrients.
- Our cQrex® peptides help improve titer, boost productivity and get the most out of cell culture processes.
cQrex® ingredients are readily available at commercial scale, with a long history of use across a variety of commercial biologics programs.
The cQrex® effect: enhanced cell culture performance
Tyrosine, cystine and glutamine are examples of amino acids that are hardly soluble or chemically reactive and are unstable. These properties limit process performance, especially if highly concentrated feed or perfusion media are required to intensify the process.
cQrex® dipeptides from Evonik provide an elegant solution. Chemical coupling to carrier amino acids yields dipeptides that are stable and highly soluble at neutral pH. These natural building blocks are efficiently utilized by a variety of animal and human cells.
SPICE UP YOUR MEDIA FORMULATION WITH THE CQREX® INGREDIENTS
| Product group | Product | Application |
|---|---|---|
| Glutamine peptides | cQrex® AQ L-Alanyl - L- Glutamine |
Classical stabilized source of L-glutamine, with a fast uptake rate |
| cQrex® GQ Glycyl-L-Glutamine hydrate |
Alternative glutamine peptide for cell lines which are sensitive to L- alanine accumulation |
|
| Tyrosine peptides | cQrex® GY Glycyl-L-tyrosine dihydrate |
Soluble source of L-tyrosine, when high concentrations at neutral pH are needed |
| cQrex® AY L-alanyl-L-tyrosine dehydrate |
Alternative tyrosine peptide for more flexibility in media design |
|
| Cystine peptide | cQrex® AC N, N'-di-L-Alanyl-L-Cystine |
Soluble source of L-cystine for concentrated feeds |
| Additives | cQrex® AKG a - Ketoglutaric acid |
Titer-enhancing additive to protect cells against oxidative damage |
| cQrex® Carnosine B-Alanyl-L-Histidine |
Antioxidant peptide to influence cell aging in primary and stem cells |
PHYTOCHOL® BIOPHARMA: A PHYTOCHEMICAL POWDER TO OPTIMIZE YOUR CELL CULTURE
Supplementing a cell culture medium with cholesterol can improve cell culture outcomes or even be essential for some cell lines. Chemically defined cell culture media that do not use fetal bovine serum (FBS) and natural lipids often require the addition of cholesterol to help improve cell growth, protein production, virus production and infectivity of produced viruses. Evonik recognizes the need to provide animal-free cell culture solutions and offers PhytoCholo® BioPharma for use in a wide range of cell lines including NSO cell culture for protein production, culture of insect cell lines and culture of packaging cell lines for virus production.
THE HIGHEST QUALITY AMINO ACIDS PROVIDE YOU WITH RELIABLE CELL CULTURE MEDIA PERFORMANCE
If you are looking for the highest quality cell culture ingredients, you must start with high-quality amino acids. Evonik has been a global leader in the supply of highly pure amino acids for several decades. Here are some of the reasons major media manufacturers and biopharmaceutical companies rely on Evonik as their source of cell culture solutions.
- Our amino acids are non-animal derived and precisely controlled to eliminate impurities including endotoxins and heavy metals.
- As the basis for our cell culture ingredients, our amino acids are fully compliant with CGMP requirements including the use of strict change control policies
- To further reduce regulatory risk, our well-established CGMP manufacturing facilities in Europe and Asia are ISO certified (9001, 14001), and have an excellent inspection record for audits.
D-Galactose and D-Mannose
Fine-tune your cell culture outcomes with specialty carbohydrates
PROVIDING A BALANCED ENERGY SUPPLY
The monosaccharides D-Galactose and D-Mannose are converted at a reduced rate by many cells and can be used to replace D-Glucose partially or fully. This can result in a more balanced primary metabolism, reduced side product formation and an optimized cell culture performance, e.g. for production of therapeutic proteins like antibodies.
BUILDING BLOCKS FOR OPTIMIZING PROTEIN QUALITY
In addition to providing an energy source, sugars are crucial building blocks for protein glycosylation. Obtaining the right glycosylation profile is a key product quality feature for recombinant proteins, and limitations in intracellular glycosylation precursors can negatively impact product quality. It has been shown that D-Galactose and D-Mannose can be used to tune the glycosylation profile of therapeutic proteins in mammalian cell culture.
About EVONIK:
Evonik is one of the world’s leading specialty chemical companies and a global partner to the pharma and biotech industry, with over 33,000 employees from more than 100 countries active across 170+ sites. Pharmaceutical and biotech customers from around the world leverage our broad portfolio of cell culture ingredients and value-adding services. We address complex customer demands for high quality cell culture solutions by relying on decades of expertise in the production of amino acids and our strong backward integration at trusted manufacturing sites in Europe and Asia.
