BEST PRACTICES FOR NGS SAMPLE PREPARATION

Next-Generation Sequencing (NGS) technologies has demonstrated the ability to sequence DNA/RNA at an exceptional speed, thereby enabling us to extract genetic information from samples faster, more consistently, and at a lower cost than ever before.

Getting your DNA arranged for sequencing involves the preparation of a sequencing library and also a couple of other steps that depend on the sample type and the NGS platform. An efficient and uniform homogenization is required to obtain an adequate quantity and quality of nucleic acids.

Mechanical lysis and more specifically bead-beating technology are considered the gold standard techniques for sample homogenization.

Benefits of NGS:

• Higher sensitivity to detect low-frequency variants.
• Faster turnaround time for high sample volumes.
• Comprehensive genomic coverage.

For this reason, a 3-dimensional bead-beating technology has been selected by Bertin Technologies to empower its range of homogenizers, the Precellys homogenizer.

Download this whitepaper to discover the best practices for NGS sample preparation with Precellys homogenizer. Fill out the form to get started.