Effective mAb Aggregate Removal: Evaluating Three Key Chromatography Resins

Efficient impurity removal is critical to ensuring the quality and safety of monoclonal antibodies (mAbs). While cation exchange chromatography (CEX) has traditionally been the method of choice for eliminating high molecular weight species (HMWS), increasing impurity levels have highlighted the need for more selective purification strategies.

Advanced chromatography techniques - such as hydrophobic interaction chromatography (HIC) and mixed-mode chromatography - are emerging as effective alternatives.

In this webinar, we present findings from a study on an IgG1 post-Protein A pool, evaluating the aggregate removal efficiency of three chromatography resins. Gain valuable insights into optimizing downstream processing and selecting the most suitable approach for mAb purification.

• POROS^™ XS Strong CEX Resin - Ion exchange chromatography (IEX) is a purification technique that allows for the separation of molecules based on their surface charge. 
• POROS^™ Benzyl Ultra HIC Resin - Hydrophobic interaction chromatography (HIC) is a versatile method for the purification and separation of biomolecules by using the function of hydrophobicity. 
• POROS^™ Caprylate Mixed-Mode CEX Resin -Mixed-mode chromatography is a versatile separation technique that utilizes multiple modes of separation to achieve greater selectivity and specificity in separating complex mixtures of biological molecules.