The need for high-throughput sample acquisition and automation in flow cytometry creates demand for new methods for analytical standardization and for reducing sources of variability. Sources of variation in flow cytometry experiments include differences in sample preparation, user uncertainty, differences in biological samples, cell analyzer performance, and instrument acquisition settings. Additionally, differences in how users gate populations and analyze the same data within an experiment are major sources of variability. Researchers often debate about the best gating strategies, and variations in such strategies may often lead to poor assay standardization. Standardization is crucial for obtaining reproducible results and ensuring that experimental data support research conclusions.

Attune CytPix Flow Cytometer:

The Invitrogen™ Attune™ NxT Flow Cytometer is a clog-resistant, workhorse cell analyzer that can be configured with up to four spatially separated lasers for analysis of up to 14 fluorescence parameters. Its unique acoustic-assisted hydrodynamic focusing feature enables sample flow rates up to 1,000 µL/min and acquisition speeds up to 35,000 events/sec, making it one of the fastest benchtop cell analyzers on the market. The Invitrogen™ Attune™ CytPix™ Flow Cytometer comes equipped with all the features of the Attune NxT Flow Cytometer. In addition, it has a high-speed bright-field camera that records image events as they pass through the flow cell to verify that cell populations consist of single cells and to establish cell morphology. The Attune CytPix Flow Cytometer captures up to 6,000 high-resolution images/second and particles as small as 0.8 μm. A variety of particles and cell types can be reliably imaged, analyzed, and correlated to their respective events on bivariate plots, providing flexibility for developing a wealth of biological applications