Difco TC Yeastolate Ultra-Filtered (TCY UF) in scale-up optimization

A customer asked us to replace a chemically defined CHO medium with a higher-performing medium and feed pairing strategy to optimize their results. The goal of this study was to meet or exceed the current baseline titer (control) established in bioreactors. A basal medium and feeds were developed after traditional spent media analyses. Following development, a variety of peptones were tested in the basal medium and as feed supplements to improve performance. Process development work was also done to optimize productivity and confirm results in a bioreactor platform.