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Ultrapure Water for HPLC Analysis

The use of a highly pure mobile phase is essential for ensuring the highest analytical-grade quality. The eluents must be especially pure in terms of their physical and chemical properties and must not contain any organic impurities or particles.

Achieve Consistent Baselines and Fewer Ghost Peaks with Fresh Ultrapure Water

The use of a highly pure mobile phase is essential for ensuring the highest analytical-grade quality. The eluents must be especially pure in terms of their physical and chemical properties and must not contain any organic impurities or particles. Tests have proven that up to 80% of the problems occurring during HPLC runs are attributable to inadequate water quality. Even purchased HPLC-grade water is frequently found to have a high total organic carbon (TOC) level.

The arium® ultrapure lab water systems from Sartorius provide Type 1 water that is RNase, DNase, and endotoxin free. These systems guarantee on-demand, reagent-grade water that meets all requirements according to the ASTM, NCCLS, ISO and USP standards—without the need to find storage for numerous water bottles.

Read this application note to evaluate an ultrapure lab water systems as an alternative to commercially sold ultrapure water for the preparation of high-purity eluants for HPLC analysis.

Using Sartorius products to prepare samples for HPLC prevents many common problems and permits higher analytical accuracy.

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